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Intra‐familial distribution of Fusobacterium nucleatumstrains in healthy families with optimal plaque control

Identifieur interne : 009400 ( Main/Exploration ); précédent : 009399; suivant : 009401

Intra‐familial distribution of Fusobacterium nucleatumstrains in healthy families with optimal plaque control

Auteurs : G. Suchett-Kaye [France] ; D. Décoret [France] ; O. Barsotti [France]

Source :

Journal of Clinical Periodontology [ 0303-6979 ] ; 1999-06.

RBID : ISTEX:8D95F84CE8F328C7CEB2B1369E20ECEA289ECD7F

English descriptors

Abstract

Abstract. Fusobacterium nucleatum, a Gram‐negative anaerobic rod associated with periodontal disease, is also found in healthy individuals and is considered part of the indigenous oral microflora. Although intra‐familial transmission of periodontal pathogens has been documented, there are no data relating transmission of F. nucleatum. This study investigated the distribution of F. nucleatum strains in 4 strictly healthy families. 32 F. nucleatum strains were isolated from 19 individuals (8 parents and 11 children aged 1–13 years). DNA was extracted and digested with the restriction endonucleases EcoRI, TaqI and HindIII. The digests were separated by electrophoresis through 0.8% agarose gels at 40 V overnight, in TBE buffer containing 1 μg/ml ethidium bromide, and photographed. The DNA was transferred to nylon filters by Southern blotting and hybridized with a digoxigenin labelled E. coli rRNA probe (Kit Dig DNA Labelling mixture – Boehringer). Probed DNA was visualized colorimetrically (CSPD Luminescent Detection Kit Boehringer) and photographed (Amersham). We found that 10/11 children shared identical ribotypes with at least one of their respective parents. Some of the children also harbored a unique additional ribotype. On the basis of indistinguishable restriction endonuclease and ribotype patterns these results support the hypothesis that intra‐familial transmission of F. nucleatum is possible.

Url:

https://api.istex.fr/document/8D95F84CE8F328C7CEB2B1369E20ECEA289ECD7F/fulltext/pdf

DOI: 10.1034/j.1600-051X.1999.260611.x

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract. Fusobacterium nucleatum, a Gram‐negative anaerobic rod associated with periodontal disease, is also found in healthy individuals and is considered part of the indigenous oral microflora. Although intra‐familial transmission of periodontal pathogens has been documented, there are no data relating transmission of F. nucleatum. This study investigated the distribution of F. nucleatum strains in 4 strictly healthy families. 32 F. nucleatum strains were isolated from 19 individuals (8 parents and 11 children aged 1–13 years). DNA was extracted and digested with the restriction endonucleases EcoRI, TaqI and HindIII. The digests were separated by electrophoresis through 0.8% agarose gels at 40 V overnight, in TBE buffer containing 1 μg/ml ethidium bromide, and photographed. The DNA was transferred to nylon filters by Southern blotting and hybridized with a digoxigenin labelled E. coli rRNA probe (Kit Dig DNA Labelling mixture – Boehringer). Probed DNA was visualized colorimetrically (CSPD Luminescent Detection Kit Boehringer) and photographed (Amersham). We found that 10/11 children shared identical ribotypes with at least one of their respective parents. Some of the children also harbored a unique additional ribotype. On the basis of indistinguishable restriction endonuclease and ribotype patterns these results support the hypothesis that intra‐familial transmission of F. nucleatum is possible.</div>
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Intra‐familial distribution of Fusobacterium nucleatumstrains in healthy families with optimal plaque control

Intra‐familial distribution of Fusobacterium nucleatumstrains in healthy families with optimal plaque control

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri